Fig. 5. Genistein reduces interaction of HOTAIR with SMARCB1. (a) SMARCB1 expression was analyzed by Western blot in 786-O and ACHN cells treated with genistein (25 μM) or control (DMSO) for 96 hours. (b) 786-O and ACHN cells were transfected with control vector (pcDNA3.1(+)) or MS2-tagged HOTAIR vector and RNA pull-down assays were performed. Pulled-down SMARCB1 was detected by Western blot. (c) SMARCB1 from 786-O and ACHN cells cultured with genistein (25 μM) or control (DMSO) for 48 hours were pulled down using anti-SMARCB1 antibody. HOTAIR associated with SMARCB1 proteins was detected by quantitative RT-PCR. Data from quantitative RT-PCR are presented with respect to IgG that is set to a value of 1. (d) Immunoprecipitated proteins were analyzed by Western blot. **p<0.01, ***p<0.001.